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2 edition of Serological, genomic and biological characterisation of equine rotavirus VP4 proteins found in the catalog.

Serological, genomic and biological characterisation of equine rotavirus VP4 proteins

Pavel IsМЊa

Serological, genomic and biological characterisation of equine rotavirus VP4 proteins

by Pavel IsМЊa

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  • 32 Currently reading

Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1994.

StatementPavel Iša.
The Physical Object
Pagination v (f2)
ID Numbers
Open LibraryOL20903638M

Abstract. Rotaviruses (RVs) are highly important pathogens that cause severe diarrhea among infants and young children worldwide. The understanding of the molecular mechanisms underlying RV replication and pathogenesis has been hampered by the lack of an entirely plasmid-based reverse genetics laikipiatourism.com by: Rotavirus/coronavirus, bovine/equine - PCR. Includes Coronavirus - PCR, Rotavirus Group A and B - PCR.

Genetic analysis of the porcine group B rotavirus NSP2 gene of Medical and Biological Research is partially financed by. laikipiatourism.com Braz J Med Biol Res 43(1) Brazilian Journal of Medical and Biological Research () ISSN X Short Communication Genetic analysis of the porcine group B rotavirus NSP2 gene from. Virus induced enteritis is one of the serious problems accounting for maximum deaths in neonatal animals and human throughout the world. The absence of appropriate surveillance programs and laboratory facilities have resulted in a scarcity of accessible data on virus associated diarrheas (especially rotavirus) in pigs in the East African region.

b Center for Equine Health, School of Veterinary Medicine, University of California, Davis, CA , USA Accepted 8 August Abstract Equine herpes myeloencephalopathy (EHM), although a relatively uncommon manifestation of equine herpesvirus-1 (EHV-1) infec-tion, can cause devastating losses on individual farms or boarding stables. Restriction fragment length polymorphism analysis of rotavirus VP7-encoding gene from humans and animals of Northeast India: a relative study of Indian and global isolates - Volume Issue 12 - P. CHAKRABORTY, N. N. BARMAN, I. SHARMACited by: 1.


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Serological, genomic and biological characterisation of equine rotavirus VP4 proteins by Pavel IsМЊa Download PDF EPUB FB2

Serological and genomic characterisation of group A rotaviruses from lambs and G Their corresponding P types were P1, P11, and P14 respectively. The only previous isolation of a rotavirus with VP4 of type P14 was also from lambs.

The fourth isolate was G9P8, which is the first record of a G9 rotavirus from a species other than man Cited by: Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus.

Woode GN, Greenberg HB, Rao CD. Both surface proteins (VP4 and VP7) of an asymptomatic neonatal rotavirus strain (I) have high levels of sequence identity with the homologous proteins of a serotype 10 bovine rotavirus Cited by: Since type specificity of the outer capsid proteins of a rotavirus appears to play an important role in protection against disease in experimental animal models, continued efforts have been made for classification and typing of neutralization specificities on the VP7 or VP4 capsid laikipiatourism.com by: Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus.

Pongsuwanna Y(1), Taniguchi K, Chiwakul M, Urasawa T, Wakasugi F, Jayavasu C, Urasawa S. Author information: (1)Department of Medical Sciences, Cited by: A novel type of VP4 carried by a porcine rotavirus strain. LiprandiSerological and genomic characterization of two porcine rotaviruses with serotype G1 specificity.

D.R. SnodgrassSerological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes. Virology, (), pp. Cited by: Aug 17,  · Evidence for the porcine origin of equine rotavirus strain H P. Iša, D.R. SnodgrassSerological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes.

Virology, (), pp. Google laikipiatourism.com by: Aug 17,  · Read "Evidence for the porcine origin Serological equine rotavirus strain H-1, Veterinary Microbiology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

Genomic Characterization of Porcine Rotaviruses in Italy yet antigenic and molecular properties of the outer capsid proteins, VP4 (a protein whose cleavage is required for infectivity.

Determination of Porcine Rotavirus Serotypes by RT-PCR and RFLP Analysis (G5) and YM (G11) rotavirus with five different restriction endonucleases are shown in Fig. 1-A. For VP4, digestion pattern of reference PoRV strains, Gottfried (P2B), OSU (P9) and YM (P9) is shown in Fig.

1-B. RFLP patterns of VP7 and VP4 gene from field PoRV. Genetic mapping indicates that VP4 is the rotavirus cell attachment protein in vitro and in vivo Article (PDF Available) in Journal of Virology 70(1) · January with 35 Reads.

Sep 01,  · The 2 outer capsid proteins, VP4 and VP7, play an important role by stimulating the production of neutralizing antibody [15, 16]. However, antibody response after rotavirus infection is broader and comprises antibody against various other rotavirus proteins, including VP2, VP6, NSP2, and NSP4.

Increasing evidence suggests that viral proteins Cited by: Antigenic and biochemical characterization of bovine rotavirus V, a new member of rotavirus serotype 10 Harald Briissow, 1. V and a reassortant rotavirus containing the VP4 from BRV UK. In addition the major tryptic cleavage product of VP4, VP5*, from BRV V is a serological and biochemical description of BRV V (Bachmann.

Rotavirus is a virus that causes laikipiatourism.com of the time only children who are younger than five years old get the disease.

The signs of the disease are severe diarrhoea and vomiting, which start suddenly and last for two or three days. Rotavirus also causes diarrhoea in animals. A vaccine, which prevents children from getting the disease is available in many laikipiatourism.com: Group III (dsRNA).

Mar 22,  · Rotavirus is approximately 75 nm in diameter and has multilayered icosahedral protein capsid composed of an outer layer, an inner layer, and a core. The virus genome is comprised of 11 segments of double-stranded RNA, encoding six structural proteins (VP1-VP4, VP6, VP7) and six nonstructural proteins (NSP) [5, 6].Cited by: 9.

rotavirus VP4 proteins expressed by baculovirus recombi-nants (Gorziglia et al., ). A criterion used to establish a P serotype in that study was an eightfold difference between homologous and heterologous reciprocal neutralizing anti-body titers. A P subtype (P1A and P1B) was established by.

2 rotavirus strains are part of a novel group of rotaviruses. We determined complete nucleotide sequences of the 4 RNA segments encoding viral protein 4 (VP4), VP6, VP7, and nonstructural protein 4 (NSP4) of a porcine rotavirus strain (SKA-1) from Japan (12) by using cDNA products obtained by a single-primer amplifi cation method (13,14).

diarrea, rotavirus bovino, técnicas, VP4, Yaracuy) report of a strain of bovine rotavirus genotype P[5], which circulates in the States of Yaracuy, Venezuela, and can serve as a support for the inclusion of this viral group to the vaccines that are being used in the country.

(Key words: Analysis, genotypes, proteins. 1) (18, 52, 53, 76, 78). VP7 is a glycoprotein, and VP4 is proteolyzed into VP8 and VP5. These modifications of the outer capsid proteins are referred to as the G and P proteins in serotype nomenclature of rotavirus, which is based on the combinations of G and P type proteins present in the outer capsid, that determine their antigenicity.

May 23,  · Bovine rotavirus (BRV) is a non-enveloped dsRNA virus that cause neonatal calf diarrhea. Lipid rafts are cholesterol-enrich membrane mircodomains that play a vital role in many cellular processes. In this study, the effect of cellular cholesterol depletion on infection of MA cells with bovine rotavirus was laikipiatourism.com by: 4.

Genetic analyses have contributed significantly to our understanding of the biology of the rotaviruses. The distinguishing feature of the virus is a genome consisting of 11 segments of double-stranded RNA. The segmented nature of the genome allows reassortment of genome segments during mixed infections, which is the major distinguishing feature of rotavirus genetics.

Reassortment has been a Cited by:. Oct 01,  · Origin of virus strains. The bovine K5 and porcine K71 RVA strains were isolated from diarrheic fecal samples of a piglet and a calf, respectively [11, 32].Both strains were passaged eight times in a monolayer of TF cells (a cloned derivative of MA monkey kidney cells) including the initial adaptation and triple plaque purification prior to laikipiatourism.com by: Characterization of Rotavirus Strains with Unusual Electrophoretic Profiles Three of the virus structural proteins (VP4, gene 4; VP6, gene 6 confirm the hypothesis that the four previously characterized as avian-like rotavirus strains are in fact human rotavirus strains which underwent genomic rearrangement (Y Hoshino & AZ Kapikian Aug 06,  · Read "Comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves, Veterinary Microbiology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.